Laboratory for honey and other bee products analyses

Laboratory for honey and other bee products analyses is established in 2000 within the Department of Food and Nutrition Research. The initial aim of Laboratory was performance of analyses of honey for the purposes of the national contest in collaboration with the Croatian Chamber of Economy, County Chamber Osijek and Croatian Beekeepers Association. In March 2005 the Ministry of Agriculture, Forestry and Water Management appointed the Department's Food Quality Laboratory as the official laboratory for honey and other bee products analyses (N.N. 41/2005). Since then, besides performing the analyses for national contest and the beekeepers, a number of scientific research and projects have been launched within the Sub-department of Food Quality. For the last fifteen years, the scientific and professional interest of Sub-department employees is mostly related to the honey and other bee products. Through IPA project “Panonian Bee”, which was held by the Osijek-Baranja County, the Laboratory received valuable equipment that enabled a significant increase in the number of parameters analysed.

Honey analyses
Melissopalynological analysis 
Water content
Ash content
Electrical conductivity
Acidity and pH
Carbohydrate composition (HPLC method)
Diastase activity (DN)
HMF content
Water-insoluble  content
Proline content
Invertase activity 
Specific rotation
Colour 
Total phenolic content
Antioxidant capacity (DPPH)
Antioxidant capacity (FRAP)

Bee venom analyses
Melittin content

Royal jelly analyses
(2E)-10-hydroxydec-2-enoic acid content
Water content
Protein content
Acidity and pH
Carbohydrate composition (HPLC method)

Bee pollen analyses
Melissopalynological analysis 
Water content
Ash content
Fat content
Protein content
Carbohydrate composition (HPLC method)
Total phenolic content
Total flavonoid content
Antioxidant capacity (FRAP)

• DR-A1 ATAGO digital Abbe refractometer
• pH meter - Mettler Toledo
• High Pressure Liquid Chromatograph (HPLC) with Refractive Index Detector (RI) and Photodiode Array Detector (PDA), SHIMADZU
• Conductometer - Mettler Toledo
• Sigma 2-16 Centrifuge
• Spectrophotometer, SHIMADZU, UV-1800
• Lovibond comparator
• Polarimeter
• Laboratory water bath 
• Muffle furnace
• Laboratory magnetic stirrer  - Rotamix
• Technical balance - Mettler Toledo
• Analytical balance Mettler Toledo – AB204-S
• Rotary vacuum evaporator Heidolph Instruments GmbH & Co. KG
  

Ljiljana Primorac, PhD, full professor

Phone: 031/224-323

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Ivana Flanjak, PhD, associate professor

Phone: 031/224-394

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